PE101009 T HESCs 人正常子宫内膜间质细胞.pdf
名称 Cell name 描述Description
种属Species 组织Tissue
形态
Morphology
安全性
Safety
培养基
Culture Medium
PE101009
T HESCs
T HESCs are hTERT-immortalized cells exhibiting fibroblast morphology that were isolated from the uterus of a female patient with non-malignant myomas. This cell line has applications in drug development, high-throughput screening, and quality control.
Homo sapiens, human Uterus; Endometrium
Fibroblast
所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12 medium with 3.1 g/L glucose and 1mM sodium pyruvate and without phenol red supplemented with 1.5 g/L sodium bicarbonate, 1% ITS+ Premix (Report RE1211), 500ng/mL puromycin, 90%; charcoal/dextran treated fetal bovine serum , 10%
Temperature 37°C Atmosphere 95% Air, 5% CO2
Cell Thawing
注意:低温保存的细胞非常脆弱,请将冻存管放入37℃的水浴中解冻,尽快复苏细胞。
1. 在无菌区准备好15ml 离心管和T-25 培养瓶分别加入约2ml 和7ml 培养基。
2. 将冻存管放入37℃水浴中,握住冻存管晃动,直到内容物完全融化。立即将冻存管从水浴中取出,擦干并喷洒75%乙醇,移至无菌区。
3. 小心地拆卸盖子,不要碰到里面的螺纹,用移液枪轻轻吸出细胞,加入到准备好的15ml 离心管中
1000rmp, 5min 离心。
4. 弃上清,轻弹管底将细胞弹散,加入约1ml 培养基重悬细胞并转入T-25 培养瓶中,轻轻摇动培养瓶使细胞均匀分布。如有必要,松开阀盖,以便气体交换。
5. 将培养瓶放入CO2 培养箱中培养。
过夜后,观察细胞形态和数量,及时补充培养基(补液量不要超过原体积)。